Interfacial Analysis of chitosan/bone: cortical and cancellous bone matrices

Abstract

Thermal stability and phase separation of a film on a substrate can be controlled at the interface level by changing the compatibility between both components, which is based on molecular interactions. Thus, in this study chitosan was used as a coating agent due to its superior film-forming properties and multiple molecular interactions with bone. In particular, two types of bone substrates were considered to study the bio-adhesion phenomena: cancellous and cortical bones. Firstly, chitosan/bone samples were prepared by controllable dip-coating method under fixed conditions, and subsequently an experimental investigation was utilized to analyze the interfacial compatibility and interaction between chitosan with the two bone substrates. As a result, Fourier transform infrared spectroscopy (FTIR) data revealed a strong interaction between the chitosan molecules and bones. Meanwhile, by scanning electron microscope (SEM) and microhardness analysis a moderate interfacial compatibility was exhibited. Furthermore, analyses by X-ray diffraction were used to identify the spatial arrangement of chitosan structure on bone, which was increased as a result of the increased acidity of chitosan solution